Use: This assay uses LC/MS-MS to quantify the free acid, glycine-conjugate and taurine-conjugate forms of cholic acid, chenodeoxycholic acid, deoxycholic acid, and ursodeoxycholic acid. Bile acids may be measured to investigate obstructive cholestasis in pregnancy.1 In addition, bile acids are required for cholesterol absorption, fat-soluble vitamin absorption, and to provide resistance to the overgrowth of intestinal bacteria.1 Bile acid levels may be reduced or elevated due to various genetic defects.

Methodology: High-pressure liquid chromatography/tandem mass spectrometry (HPLC/MS-MS)