Oxidative Stress 2.0 (Urine)

Why use the Oxidative Stress 2.0 (Urine) Profile?

Oxidative Stress Analysis 2.0 is a nutritional test used to evaluate the body's oxidative stress status. Normal human metabolism produces highly reactive molecules known as free radicals, typically controlled by antioxidants. When production of free radicals becomes excessive, the antioxidant mechanisms of the cell become overwhelmed, resulting in oxidative damage. The Oxidative Stress 2.0 (Urine) uses a simple urine test to evaluate two key biomarkers of oxidative stress damage.

Effective for Patients Experiencing:

Since oxidative stress can affect cellular function in many body systems, it has been associated with numerous conditions including:

• Chronic Pain/Inflammatory Conditions
• Cardiovascular Disease
• Aging and Neurodegenerative Conditions

About the Oxidative Stress 2.0 (Urine) profile

Biomarkers of oxidative damage can be found in the urine. The Oxidative Stress Analysis 2.0 (Urine) measures these compounds:

• Lipid Peroxides – reflective of oxidative damage to polyunsaturated fatty acids (components of cell membranes)
• 8-OHdG – reflective of oxidative damage to DNA The Oxidative Stress 2.0 Urine test results are displayed using colorimetric reporting, allowing a quick determination of patient need for antioxidant support.

What is the Oxidative Stress 2.0 (Urine) Profile?

The Oxidative Stress 2.0 (Urine) evaluates two key biomarkers for oxidative damage. In the course of normal human activity — energy production, detoxification of environmental pollutants, and immunologic defense mechanisms — highly reactive molecules known as free radicals are produced. This effect is naturally counterbalanced in a controlled manner by the body's antioxidant systems and the dietary antioxidants found in foods.

When production of free radicals becomes excessive, the antioxidant mechanisms of the cell become overwhelmed and free radical action is not controlled, leading to impaired cell function. Oxidative stress is a condition in cells where excessive production of free radicals damages cellular components. Potential targets include lipid membranes, proteins (leading to enzyme inactivation or receptor malfunction), and DNA (leading to mutations and potential cancers).

Oxidative Stress 2.0 (Urine) uses a simple urine test to evaluate two key biomarkers of oxidative stress:

• Lipid Peroxides – reflective of oxidative damage to polyunsaturated fatty acids (components of cell membranes)
• 8-OHdG – reflective of oxidative damage to DNA

The Oxidative Stress 2.0 Urine test results are displayed using colorimetric reporting, allowing a quick determination of patient need for antioxidant support.

What Advantage Does the Oxidative Stress 2.0 (Urine) Profile Offer Compared to Other Diagnostics?

Measurement of oxidative stress biomarkers in urine is a patient-friendly non-invasive approach to evaluating levels of damage being produced by free radicals and the need for additional antioxidant support. This profile is also a convenient tool to monitor response to therapies.

What Can Clinicians and Patients Expect from Oxidative Stress 2.0 (Urine) Testing?

Assessment of urine biomarkers for oxidative-stress damage can indicate the functional need for dietary and/or lifestyle modifications and provide guidelines for customized antioxidant supplementation, which can help minimize future oxidative damage.

Performing Lab: Genova

Important things to know and consider

• Abnormal kidney function or use of diuretics may influence test results. This test should not be performed on individuals with kidney disorders. In addition, certain medicines may impact test results [e.g.cephalosporins (e.g.Cefoxitin), cimetidine (Tagamet), fibrates (e.g.Ciprofibrate) and trimethoprim-sulfamethoxazole (Bactrim)]. Let your physician know about your use of these medications. Do not change use of medications unless instructed to do so by your healthcare provider.

Schedule & Prepare for your urine collection

• Avoid over-hydration. Aim for an average overall fluid intake of ½ ounce of water/fluid per pound of body weight per day. Example: 130lbs = 65ounces (eight 8-oz glasses per day)
• Freeze the enclosed freezer brick a minimum of 8 hours before shipping.
• Contact FedEx and schedule to ship the specimen overnight delivery Monday-Friday.
• Female patients should not collect urine during a menstrual period.
• Complete the Requisition Form with all patient and billing information. Be sure it is signed by the Patient/Responsible Party and the healthcare provider. Enter date in box labeled “Date Final Sample Collected"

Ship the Specimen to the Lab

Specimen must be returned in the Genova Diagnostics kit box for correct delivery to the lab. Not following these instructions may result in a shipping charge.

• Plan to ship the specimen Monday–Friday overnight delivery.
• Call 1.800.GoFedEx(1.800.463.3339) to schedule shipping.
• Make sure the tube is tightly closed and identified with completed label. Seal the tube in the Biohazard bag.
• Lay the Biohazard bag with the urine specimen on top of the freezer brick in the foam box. Secure the foam box lid with the rubber band.
• Slide the foam box back inside the kit box, and place the completed and signed requisition on top before closing. Do not staple or tape the box.
• Print your name and address in the section marked “From” on the prepaid shipping envelope label. DO NOT mark or write in any other sections. Put the kit box into the envelope and seal the envelope.
• Keep your shipment and tracking numbers for future reference and tracking purposes.